Figure 1
From: Elevation of autophagy markers in Sjögren syndrome dry eye
Expression of Autophagy Markers ATG5 and LC3B in Tears and Conjunctival Impression Cytology Specimens from Controls, Patients with non-Sjögren Syndrome Dry Eye, and Patients with Sjögren Syndrome Dry Eye. (A). Representative Western blot images and relative densitometry data on ATG5 protein expression and the LC3B-II/I conversion ratio in tears demonstrating increases in patients with Sjögren Syndrome (SS) dry eye (DE) compared to patients with non-SS DE and controls. The protein expressions of ATG5, LC3B-I, LC3B-II and β-actin were developed from the same gel in western blot. (B). The ATG5 and LC3B-II mRNA expression levels in the conjunctiva as measured by real time PCR demonstrating increases in patients with SS DE compared to patients with non-SS DE and controls. (C). Representative confocal images of immunostained conjunctiva from impression cytology specimens showing that the punctate cytoplasmic staining patterns of ATG5 and LC3B-II indicative of autophagy were enhanced in patients with SS DE. *P < 0.05. Scale bar = 20 μm.
