Figure 2
From: Elevation of autophagy markers in Sjögren syndrome dry eye
Immunofluorescent Staining of Autophagy Markers (ATG5 and LC3B-II) in the Lacrimal Glands of Sjögren Syndrome Animal Models (NOD/LtJ mice). (A) Representative immunofluorescent photos demonstrating cytoplasmic punctate staining patterns of ATG5 and LC3B-II on the lacrimal gland developed prominently at 16 weeks of gestational age in NOD/LtJ mice. Those markers were not prominent at 8-week-old NOD/LtJ mice. Lacrimal glands of 16-week-old BALB/c mice were used as a control. DAPI (blue) was used for nuclear counterstaining. Scale bar = 20 μm. (n = 6). (B) Western blotting and relative densitometry graph demonstrates ATG5 protein expression and the LC3B-II/I conversion ratio from lysates of 8− and 16-week-old NOD/LtJ, and 16-week-old BALB/c mice. Both markers were significantly higher at 16 weeks than 8 weeks of gestational age in NOD/LtJ mice (n = 6). The protein expressions of ATG5, LC3B-I, LC3B-II and β-actin were developed from the same gel in western blot. *P < 0.0001.
