Figure 3
(A) Macroscopic, and microscopic view of H&E, immunofluorescence staining for UCP-1, fluorescence staining for mitochondria (MitoGreen), and Oil red O staining of interscapular fat depot in a ZL control, ZDF rat with no-restriction diet (ZDF-ND) and a ZDF rat with calorie restriction (ZDF-CR). In immunofluorescence staining for UCP-1 (green) and fluorescence staining for mitochondria (green) nuclei were stained with DAPI (blue) and cell membranes with wheat germ agglutinin (red). Visual analysis demonstrates a reduction in mitochondria and UCP-1 expression as well as an increase in fat cells in both ZDF-ND and ZDF-CR rats. (B) Bar graphs show weight of interscapular fat depot, size of adipocytes and percentage of positively stained area of UPC-1 and mitochondria staining in ZL control, ZDF-ND and ZDF-CR rats. *p < 0.05, **p < 0.01, ***p < 0.0001.
