Figure 4

(A) Protein expression of the thermogenic protein UCP-1 in BAT was significantly higher in Zucker Lean (ZL, n = 6) control rats in comparison to diabetic rats with (ZDF-ND; n = 6) and without (ZDF-CR, n = 5) calorie restriction. (B) Protein expression of fatty acids synthesis protein acetyl-coenzyme A carboxylase (ACC) was significantly higher in BAT of ZDF-ND rats in comparison to ZL rats; ZDF-CR presented intermediate values that did not reach a significant difference to either ZL or ZDF-ND rats. β-Actin (BACT) was used as housekeeping control. *p < 0.05.