Figure 4 | Scientific Reports

Figure 4

From: Three-dimensional organotypic matrices from alternative collagen sources as pre-clinical models for cell biology

Figure 4

Immunohistochemical (IHC) staining of embedded fibroblasts for three key stromal markers, (a) fibroblast activation protein (FAP), (b) alpha smooth muscle actin (αSMA) and (c) phosphorylated myosin phosphatase target subunit 1 (phospho-MYPT1), scored for positive cells per 500 × 500 μm region of interest (#/ROI; n = 3, scale bars: 50 μm, scale bars (insets): 10 μm). Mean ± SD. (d) Quantitative real-time PCR (qRT-PCR) analysis of relative mRNA expression, normalized to GAPDH, of genes indicative of fibroblast activity and matrix deposition (n = 4). ND – not detected. Mean ± SEM.

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