Figure 5 | Scientific Reports

Figure 5

From: Three-dimensional organotypic matrices from alternative collagen sources as pre-clinical models for cell biology

Figure 5

Organotypic invasion assays demonstrating the multiple readouts possible from several established cancer cell lines and an example patient-derived cell line (PDCL) model. Representative images and quantification are given for (a) melanoma (CHL-1, n = 3), (b) squamous cell carcinoma (SCC; A431, n = 3), (c) pancreatic ductal adenocarcinoma (PDAC; KPC, n = 3) and (d) a PDAC PDCL (n = 3). Each cell line was then scored for invasive cells (i, S100B/pan-cytokeratin, which excludes fibroblasts), proliferating cells (ii, Ki67) and apoptotic cells (iii, cleaved caspase-3). Scale bars: 100 μm. Scale bars (insets): 12.5 μm. Mean ± SEM.

Back to article page