Figure 3 | Scientific Reports

Figure 3

From: Three-dimensional (3D) culture of adult murine colon as an in vitro model of cryptosporidiosis: Proof of concept

Figure 3

C. parvum infection of the murine colonic explant system (A) Hematoxylin eosin safranin (HES) staining of an uninfected SCID mouse colon section. Scale bar, 15 µm (B) HES staining of a colonic explant section after 12 days of culture followed of 4 days after infection with C. parvum. Developmental stages of C. parvum are observed in the apical position (arrows) within the intestinal epithelial cells. Scale bar, 10 µm (C) HES staining of cultured colonic explant sections after 14 days post-infection with C. parvum showing extracellular and intracellular stages of the parasite (arrows). (D) HES staining of cultured colonic explant sections at 22 days post-infection with C. parvum showing parasites within the intestinal epithelial cells. Scale bar, 25 µm.

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