Figure 3
Detection of HAN and Hef and their co-localization in T. kodakarensis cells. (a) Estimation of the intracellular concentrations of HAN and Hef in T. kodakarensis cells. The loading amounts and detection conditions were depending on the activities of the antibodies. (b) Immunoprecipitation of HAN and Hef from the T. kodakarensis cell extract. The immunocomplexes were captured individually with each antiserum from the whole cell extract (as shown at the top), and were subjected to SDS-10% PAGE followed by western blot analyses using each antiserum (shown on the right side). The whole cell extracts without immunoprecipitation (Input) and those precipitated after a treatment with preimmune serum (PI) were also loaded as positive and negative controls, respectively. The relative loading amounts are indicated at the bottom of the panels.
