Figure 6
Sensitivities of the Δhan mutant to DNA-damaging treatments. (a) Drop dilution assays for the sensitivities of parental strain (KUW1), Δhan, and Δhef cells to 93 °C, MMC, and UV-C. The serially diluted T. kodakarensis cells (5 × 105, 5 × 104, 5 × 103, 5 × 102, 5 × 101, and 5 × 100 cells from left to right) were spotted on the plates before the treatment, as described in the Materials and Methods. The plates were then incubated anaerobically at 85 °C [“No damage”, “MMC”, and “UV (5 J/m2)”] and 93 °C (“93 °C”). (b) Quantifications of the rates of surviving colonies from (a) were plotted from more than nine independent experiments using Kaleida Graph 4.5 (Synergy Software). The value of each bar depicts a mean determined from calculated plots. (c) The survival fraction curves (treated with 5, 8, and 11 J/m2 UV-C dose) of different strains were plotted. Each strain was examined at least thrice, and error bars represent SEM. Only the plus error bars are indicated for the Δhef strain. Symbols are indicated on the top of the panels.
