Figure 7
Proposed model of the cooperative function of HAN and Hef for stalled fork restart. The helicase and nuclease domains of Hef are drawn separately (for simplicity, Hef is drawn as monomer here). HAN works especially at the arrested fork by the delay of lagging strand synthesis (a), for which the helicase domain preferentially binds and unwinds the nascent leading strand to induce fork regression. In this case, HAN binds to the ID region of Hef. For the fork arrested by the delay of leading strand synthesis (b), the nuclease domain of Hef binds the fork junction and breaks the fork. See the text for more details.
