Figure 3
From: Identification of sRNA mediated responses to nutrient depletion in Burkholderia pseudomallei
Relative expression (in fold change value) of eleven PCR-validated sRNA candidates with respective flanking genes as determined by qRT-PCR. The differential expression (in fold change) of PCR-validated sRNA:flanking gene pairings for B. pseudomallei D286 under minimal nutrient treatment in comparison to the nutrient rich culture reference (fold changeM9/BHIB). For each pair, the checquered bars represent the fold changeM9/BHIB (log2-transformed) of putative sRNA expression, whereas the plain bars represent the fold changeM9/BHIB (log2-transformed) of putatively-interacting flanking gene expression. The relative expression (mean ± sem) of each sRNA candidate and flanking gene were normalised to dnaK using ΔΔCT method. Using statistical one sample t-test (H 0  = 1), any fold change of sRNA or gene expression that scored p-value of <0.05 is indicated with asterisk (*).
