Table 1 Summary of expression patterns between sRNA and its flanking gene(s) based on differential expression using RNA-seq and qRT-PCR analyses. Using RNA-seq and qRT-PCR analyses, the differential expression of both sRNA and its flanking gene(s) in Bp D286 under M9 growth condition in comparison to BHIB (reference condition) was summarised in the table using the designation of expression pattern as: (i) ‘up’ = up-regulated, (ii) ‘down’ = down-regulated, (iii) ‘nd’ = not detected, or (iv) ‘na’ = not available due to non-detection of upstream sRNA. The grey shades represent the congruency in terms of expression pattern of sRNA:flanking gene pairings between the two column analyses.
From: Identification of sRNA mediated responses to nutrient depletion in Burkholderia pseudomallei
cis-sRNA | Locus tag of target gene(s) | RNA-seq | qRT-PCR | ||
|---|---|---|---|---|---|
sRNA | gene(s) | sRNA | gene(s) | ||
BPNC10044R | bpsl0591 | up | up | up | up |
BPNC10113R | bpsl1725 | up | up | up | up |
Candidate_369_SIPHT | bpss1184 | up | up | nd | na |
BPNC10048R | bpsl0671, bpsl0672 | down | down, down | up | nd, up |
BPNC10196F_1129_SIPHT | bpsl3221 | down | down | up | down |
BPNC10209R | bpsl3223 | down | down | down | down |
BPNC10146R | bpsl2159 | down | down | down | down |
BPNC10037R | bpsl0493, bpsl0494 | up | up, up | up | up, up |
BPNC10233R_28_SIPHT | bpsl3418 | up | up | up | up |
Candidate_468_SIPHT | bpss1953 | up | up | nd | na |
BPNC10134F | bpsl2035 | down | down | down | down |
BPNC10146F | bpsl2187 | down | down | up | up |
