Figure 2
Quantification of the pair-wise interactions of SIRT2, TPPP/p25 and tubulin by ELISA. (a) Saturation curves of TPPP/p25 (●) and tubulin (○), when the plate was coated with SIRT2; and that of tubulin (▲), when the plate was coated with TPPP/p25. Concentrations of the coated proteins were 5 μg/ml. The data are presented as mean ± SD, n = 3 for TPPP/p25-SIRT2, n = 4 for tubulin-SIRT2, and n = 11 for TPPP/p25-tubulin. (b) Displacement of 125 nM TPPP/p25 from the coated SIRT2 by tubulin added at various concentrations detected by TPPP/p25 antibody. The data are presented as mean ± SD, n = 3. (c) Binding of TPPP/p25 (250 nM) and tubulin (1 μM) to the immobilized SIRT2 as detected by TPPP/p25 and tubulin antibodies, respectively. The data are presented as mean ± SD, n = 4. *p = 2.61E-04 when TPPP/p25 with tubulin compared to TPPP/p25 alone, and *p = 1.76E-02 when tubulin with TPPP/p25 compared to tubulin alone (two-sided, unpaired Student’s t-test).
