Figure 3
Mathematical model of the binary and ternary complexes and comparison with the ELISA experiment. (a) Concentration of the different complexes according to the binary (dashed lines) and ternary (solid lines) models. (b) SIRT2 was immobilized on the plate, then tubulin without (○) or with 200 nM TPPP/p25 (●) was added and the bound tubulin was detected by tubulin antibody. The data are presented as mean ± SD, n = 4 for tubulin, n = 3 for tubulin with TPPP/p25. SIRT2-tubulin at 0 μM TPPP/p25: relative concentration of SIRT2-tubulin complex without added TPPP/p25. Relative concentrations of SIRT2-tubulin, TPPP/p25-SIRT2-tubulin and the sum of SIRT2-tubulin and TPPP/p25-SIRT2-tubulin according to the ternary model, respectively, are shown. Relative concentration of SIRT2-tubulin according to the binary model is also displayed.
