Figure 4
Effect of SIRT2 on the TPPP/p25-induced tubulin polymerization. (a) Tubulin polymerization (6 μM) promoted by TPPP/p25 (3 μM) in the absence and presence of inactive or active (with 500 μM NAD+) SIRT2 (25 μM). The tubulin polymerization was induced by the addition of TPPP/p25 or TPPP/p25 preincubated with SIRT2. (b) Electron microscopic images of the pellet fractions produced by TPPP/p25-induced tubulin assembly in absence and presence of SIRT2 (with NAD+) (a). (c) and (d) Western blot images of supernatant (S) and pellet (P) fractions using acetyl-tubulin antibody, when polymerization was induced by TPPP/p25 premixed with SIRT2 (c) or SIRT2 was added to the TPPP/p25-assembled tubulin (d). All data of the representative experiments can be found in the source data file. The images of the full-length blots are presented in Supplementary Figs S2 and S3.
