Figure 5 | Scientific Reports

Figure 5

From: Modulation Of Microtubule Acetylation By The Interplay Of TPPP/p25, SIRT2 And New Anticancer Agents With Anti-SIRT2 Potency

Figure 5

Modest inhibitory potency of TPPP/p25 on the deacetylation of tubulin by SIRT2. (a) Concentration-dependent tubulin deacetylation by SIRT2 (●) and the inhibitory effect of TPPP/p25 (∆) on the deacetylase activity of 1.5 μM SIRT2 (indicated by an arrow). The acetylation was determined by Western blot using acetyl-tubulin antibody at 2.64 μM tubulin concentration. (b) Quantification of the Western blot data as described in the Materials and Methods. The data are presented as mean ± SD, n = 3 for the SIRT2 concentration serie, and n = 4 for the TPPP/p25 concentration serie at 1.5 μM SIRT2 concentration. Images of the full-length blots/gels are presented in Supplementary Fig. S4.

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