Figure 8
Visualization of the acetylation of the MT network produced by SIRT2 inhibitors (MZ242 and SH1 PROTAC) in TPPP/p25 expressing (green) HeLa cells as compared to the non-transfected cells by widefield immunofluorescence microscopy using specific acetyl-tubulin antibody (red). (a) Cells treated with MZ242. (c) Cells treated with SH1. Note that the chemical compounds are taken up from the medium, while TPPP/p25 is expressed only in some of the cells. Nuclei are counterstained with DAPI (blue). Scale bar: 5 μm. The acetyl-tubulin signal (red) was quantified in the transfected and non-transfected cells as described in the Materials and Methods. The data are presented as mean ± SD, at least 25 cells (in the case of MZ242, b) or 50 cells (in the case of SH1, d) were analysed. *p = 9.20E-3 and *p = 3.34E-5 when TPPP/p25 transfected cells were compared with those treated with MZ242 and SH1 as well, respectively (two-sided, unpaired Student’s t-test).
