Figure 1
The impact of ciaR mutation on biofilm formation. (A) The biofilms of WT, ΔciaR and ΔciaR/ciaR were cultured in a 96-well plate in BM media for 24 hours. Biomass was measured by crystal violet staining. (B) Biofilms of WT and ΔciaR cultured in 4-well chambers after being washed with PBS buffer. (C) Biofilms grown in 4-well chambers were stained by SYTO9 and propidium iodide. Fluorescence (left) and differential interference microscopy images (middle) were obtained by confocal laser scanning microscopy. Fluorescence images were analyzed by COMSTAT script, and heat maps of biofilm thickness were generated, which showed the distribution of biomass in biofilms (right). (D) The fluorescence images were analyzed by COMSTAT. Biofilm biomass, average thickness, propidium iodide signal and roughness coefficient were quantified, respectively. All the data in Fig. 1D were compared with their WT control. *P ≤ 0.05, **P ≤ 0.01, Student’s t-test. Means and standard deviations from triplicate experiments are shown.
