Figure 2
Polysaccharide production and cell growth of WT and ΔciaR. (A) Strains were incubated in BM at a shaking speed of 200 rpm for 24 hours. The arrow points a cellular auto-aggregate. (B) Strains were incubated in BM at a shaking speed of 200 rpm for 24 hours. Extracellular polysaccharide was stained by HHA-FITC and measured by flow cytometry. Arrow indicates a tail of increased staining in the WT strain. (C) WT and ΔciaR were cultured in BM. Every 30 minutes, cells were shaken for 3 minutes and OD600 was read using a platereader (Top). CFU values were determined after 16 hours of incubation (bottom). (D) Biofilms were cultured in BM for 24 hours and then treated with 100 U/mL of DNase I for 1 hour. Microtiter dish biofilm assay was performed after DNase I treatment. Means and standard deviations from triplicate experiments are shown. ND: no significant difference, Student’s t-test.
