Figure 4
ΔciaR impacts biofilm formation by modulating the expression of arginine biosynthesis genes. (A) Biofilm biomass was examined by microtiter dish biofilm assay and at the same time, the total protein concentration was quantified by BCA assay. The biofilm formation ability was defined as biomass (OD560) divided by total protein concentration (μg/mL). (B) Biofilms of strains were grown in BM for 24 hours in 24-well plates. WIG levels were assayed. (C) qRT-PCR assay was performed to show the expression of gtfP gene in different strains or in WT cultured in BHI with different concentrations of L-arginine. (D) WT was cultured in BM with different concentrations of L-arginine. Biofilm formation ability was examined by microtiter dish biofilm assay. Means and standard deviations from triplicate experiments are shown. *P ≤ 0.05, **P ≤ 0.01, Student’s t-test.
