Figure 5
Biofilm formation of WT and ΔciaR under flow cell conditions. Strains were marked by different fluorescent reporters and then cultured in a flow cell system. Image in Fig. 5C was obtained by CLSM. Others were recorded by the fluorescence microscopy. Biofilm biomass was quantified by COMSTAT software. (A) The biofilms biomass of WT and ΔciaR in the flow cell system at different time points. (B) WT and ΔciaR were co-cultured in the flow cell channel and biofilm biomass was measured. (C) After being co-cultured for 4 days, the structure of biofilm formed by WT – ΔciaR was examined by CLSM. For each sample in Fig. 5A and B, ten images from fluorescence microscopy were obtained to calculate the means and standard deviations. *P ≤ 0.05, **P ≤ 0.01, Student’s t-test.
