Figure 6
The effect of ΔciaR on competence stimulation and the effect of CSP and competence genes on biofilm formation. The concentration of CSP was quantified by measuring the transformation frequency of a ΔcomC mutant to which was added culture supernatant (A) or cell lysate (B) of WT and ΔciaR, respectively. (C and E) biofilm formation of strains was tested by microtiter dish biofilm assay. (D) Strains were cultured in BM with different concentrations of exogenous CSP. Biofilm formation ability was tested by microtiter dish biofilm assay. Means and standard deviations from triplicate experiments are shown. ND: no significant difference, *P ≤ 0.05, **P ≤ 0.001, ND: no significant difference, Student’s t-test.
