Figure 4 | Scientific Reports

Figure 4

From: The molecular dialogue between Arabidopsis thaliana and the necrotrophic fungus Botrytis cinerea leads to major changes in host carbon metabolism

Figure 4

Expression of Arabidopsis STP and SWEET genes, and accumulation of the AtSTP13 protein in cells exposed to B. cinerea elicitation in the Millicell system. Relative expression of the 14 STP (a) and the 17 SWEET (b) genes in mock and B. cinerea treated cells. Genes that were expressed below the detection threshold are not presented. Gene expression analysis was performed by RT-qPCR and results were normalized to the plant reference gene At4g26410. Data represent mean (+/−SE) of at least 3 independent experiments. (c) Accumulation of the AtSTP13 protein in Arabidopsis cells exposed or not (mock) to B. cinerea in the Millicell system. Cells were collected at the indicated time points and proteins were extracted from microsomal fractions. Thirty micrograms of total microsomal proteins have been loaded in each lane. Protein immunoblot was performed with a polyclonal antibody raised against the AtSTP13 protein. Images belong to the same blot, with identical exposure parameters. Protein loading was visualized by staining blots with a Ponceau S solution. Asterisks represent significant differences compared to the corresponding mock condition (Student’s t-test, * P < 0.05).

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