Figure 5

Cell wall invertase activity and expression of Arabidopsis invertase-related genes in cells exposed to B. cinerea elicitation in the Millicell system. (a) CWIN activity was assayed from insoluble extracts of Arabidopsis cells elicited or not (mock) with B. cinerea. Data represent mean (+/−SE) of 3 independent experiments. (b) Relative gene expression of AtCWIN1, AtCIF1 and AtC/VIF2. Gene expression analysis was performed by RT-qPCR and results were normalized to the plant reference gene At4g26410. Data represent mean (+/−SE) of at least 3 independent experiments. AtCWIN2, -3, -4 and -5 are not presented because transcript levels were below the detection threshold. Asterisks represent significant differences compared to the corresponding mock condition (Student’s t-test, * P < 0.05).