Figure 6

Sugar content and oxygen consumption in Arabidopis cells exposed to B. cinerea in the Millicell system. Time-course analysis of soluble sugars (a) and starch (b) contents in mock and Botrytis-challenged Arabidopsis cells. The level of soluble hexoses corresponds to the total level of soluble glucose and fructose. The amounts of soluble and insoluble sugars were assessed using enzymatic assays. Data represent mean (+/−SE) of at least 3 independent experiments. (c) Rate of oxygen consumption was measured in mock and Botrytis challenged cells using a Clark electrodes. The value of the corresponding mock condition was set to 100% for each time point. Results represent mean (+/−SE) of 3 independent experiments. Asterisks represent significant differences compared to the corresponding mock condition (Student’s t-test, * P < 0.05; ** P < 0.01).