Figure 7
From: Lactation has persistent effects on a mother’s metabolism and mitochondrial function

Markers of oxidative stress in skeletal muscle for age-matched rats that did not reproduce (NR), and rats that did not (NL) or did (L) suckle their young. Data include protein level of the antioxidants (A) SOD2, (B) SOD1, (C) CAT, and (D) GPX. In addition, data include markers of oxidative damage including (E) lipid peroxidation determined by 4-HNE, and (F) protein carbonyls levels determined by using oxyblot. Representative blots are shown under the graphs (A– D) or to the right of the graphs (E and F). Images are shown as captured, protein expression was normalized to Ponceau staining, and variability between membranes was normalized by using an internal control run with each membrane. Data shown are mean ± SD (n = 5–8 per group). No significant differences detected (p > 0.05).