Figure 6 | Scientific Reports

Figure 6

From: Characterization of novel endo-β-N-acetylglucosaminidases from Sphingobacterium species, Beauveria bassiana and Cordyceps militaris that specifically hydrolyze fucose-containing oligosaccharides and human IgG

Figure 6

Characterization of Beauveria and Cordyceps ENGases. (A) Recombinant Beauveria and Cordyceps ENGases were expressed in E. coli and were purified. Protein samples (0.5 μg of each) were then subjected to SDS-PAGE using a 5–20% acrylamide gel, following which the gel was stained with CBB. (B) Effects of pH on the enzymatic activity of Beauveria and Cordyceps ENGases. The assay was carried out using 100 mM buffers at various pHs. (C) Rituximab (IgG) was incubated separately with Endo-S, Endo-CC1, recombinant Beauveria ENGase and recombinant Cordyceps ENGase overnight and subsequently analyzed by SDS-PAGE. The CBB stained gel shows the heavy chain of IgG is migrating as a protein with MW of approximately 50 kDa. (D) RNase B was incubated separately with Endo-S, Endo-CC1, recombinant Beauveria ENGase and recombinant Cordyceps ENGase overnight and subsequently analyzed by SDS-PAGE.

Back to article page