Figure 1
From: Micro-patterned agarose gel devices for single-cell high-throughput microscopy of E. coli cells
Multi-pad agarose gel pad device provides an easy-to-use platform for high-throughput bacterial microscopy. (a) Multi-pad agarose gel pad device allows a separate sample to be added to each pad for high-throughput imaging. (b) Steps in making micro-patterned multi-pad agarose gel pads. From top left: Electron beam lithography is used to generate micro-patterning on a 3 inch silicon wafer. PDMS is cast onto the wafer. Laser cut PET plastic is adhered to the PDMS using double-sided tape. Molten agarose is poured into the mould and set at 4 °C. The gel is un-moulded and cells are pipetted onto each pad. After the liquid has dried, a coverslip is added and the device is sealed and ready for imaging.
