Figure 2
From: Micro-patterned agarose gel devices for single-cell high-throughput microscopy of E. coli cells
CapsuleHotel micro-patterned multi-pad agarose device for high-throughput imaging. (a) Design for a micro-patterned agarose pad to physically segregate single cells. (b) Cells applied to capsule micro-patterned agarose pad are physically segregated. Phase contrast (left) and YFP fluorescence (right) images of DY330 ribB_Venus are shown. Automatically detected cells are circled in green. Scale bar = 10 μm. (c) A set of DY330 Venus library strains automatically imaged across this device yielded an average of 70 cells per image from 207 images. (d,e) A set of DY330 Venus library strains imaged on two separate days gave reproducible fluorescence mean (d) and noise (e). See also Table S1.
