Figure 3
From: Micro-patterned agarose gel devices for single-cell high-throughput microscopy of E. coli cells
LineHotel for tracking growing micro-colonies (a) Design for a micro-patterned agarose pad to direct micro-colony growth in straight line. Hundreds of parallel lines (0.85 μm width, 2 μm spacing) are patterned onto the agarose surface. (b) E. coli cells from an exponentially growing culture were pipetted onto 24 separate pads of LineHotel and imaged as a time-lapse at 30 °C. Phase contrast images from one pad are shown at 0 (left) and 155 (right) minute timepoints. Analysed micro-colonies are numbered 1–10. Scale bar = 10 μm. (c) The length of 5 micro-colonies (from b) vs time was obtained from the phase contrast images and fit with an exponential growth curve. (d) Doubling times for micro-colonies from c.
