Figure 8
Ag-doped TiO2 NPs induced cytotoxicity was mediated via oxidative stress. Antioxidant N-acetylcysteine (NAC) effectively prevented the oxidative stress and cytotoxicity induced by Ag-doped TiO2 NPs in HepG2 cells. Cells were treated with Ag-doped (5%) TiO2 NPs at the concentration of 100 µg/ml in the presence or absence of NAC (10 mM) for 6 or 24 h. The ZnO NPs, H2O2 or BSO were used as positive controls. (A) ROS level, (B) SOD activity, (C) GSH level and (D) MTT cell viability. Data represented are mean ± SD of three identical experiments made in three replicate. *Significant difference as compared to the control (p < 0.05). #Significant effect of NAC against NPs and positive controls (p < 0.05).
