Figure 3
From: Synthetic integrin-binding immune stimulators target cancer cells and prevent tumor formation
Analysis of effector F2. (A) Chemotaxis of human leukocytes upon stimulation with different concentrations of (PEGylated) effector. (B) Oxidative burst of human, murine and guinea pig leukocytes upon stimulation with (PEGylated) effector. In panels A and B, data of three independent experiments are averaged. (C) Immune cell infiltration in mouse skin (HE-stained sections) 24 h after subcutaneous injection of PBS buffer (top) and 100 nmol F2-2PEG (bottom) into mouse skin. Infiltrating immune cells are visible as small dark dots (scale bar 100 µm). Black arrows indicate matrigel depots under the skin. (D) Cytokine release from human monocytes upon stimulation with F2-2PEG or LPS (averages of two independent experiments with monocytes from different donors).
