Figure 4
From: Synthetic integrin-binding immune stimulators target cancer cells and prevent tumor formation
Analysis of binder B9. (A) Structure of B9-Biotin. (B) Concentration-dependent binding of B9-Biotin to A431 (epidermoid carcinoma cells) expressing integrin α3β1 in the presence and absence of Mn2+ to induce the high affinity state of the integrin. Data was fitted based on a one site-specific binding model and gave apparent KD values of 90 nM in the presence of Mn2+ and 176 nM in its absence. (C) Displacement of an anti-CD49c-PE conjugate by increasing concentrations of B9. No displacement of the antibody occurred with scrambled binder (sc-B9). An isotype control with mlgG1-PE was used to determine background signal.
