Figure 3

STAP1 knockdown does not affect leukemic cells survival. Nalm6 and Kasumi-2 cells were transduced via spin-infection with shRNAs targeting STAP1 or scrambled control vectors. Values represent mean ± SEM of four independent experiments. Independent sample T-test. **p < 0.01; *p < 0.05. (a) Knockdown efficacy was determined three and seven days after transduction using RT-qPCR. STAP1 expression relative to RPS20 was calculated. Relative expression values towards the two scrambled controls are depicted. (b) Proliferation of Nalm6 and Kasumi-2 cells was measured for four days. At day 0 (3 days after transduction) cells were plated at equivalent concentrations. The next four days cell concentrations were detected using the MACSQuant and PI staining. Cell numbers (x10⁶) are shown on the y-axis. (c) Seven days after transduction, viability of the cells was determined using AnnexinV and PI staining. Viability relative to scrambled control samples is shown.