Figure 7 | Scientific Reports

Figure 7

From: High STAP1 expression in DUX4-rearranged cases is not suitable as therapeutic target in pediatric B-cell precursor acute lymphoblastic leukemia

Figure 7

STAP1 expression and DUX4-rearrangements. RNAseq analyses were used to determine DUX4 and STAP1 expression levels in an independent BCP-ALL cohort (n = 2 KMT2A-rearranged, n = 1 BCR-ABL1, n = 21 B-other, n = 17 ETV6-RUNX1, n = 21 high hyperdiploid). Fastq-files with paired-end data were aligned to one DUX4 gene and the STAP1 GRCh37 reference sequence using STAR 2.5.0b. Read counts were determined with HTSeq-count version 0.6.1p1. Grey lines represent the median expression values. (a) DUX4 expression values are depicted as Fragments Per Kilobase per Million mapped (FPKM). DUX4-partner genes were identified using NCBI blast, in which unknown sequences after the breakpoint were aligned52. DUX4-allignement of 4 DUX4-rearranged cases and 4 non-DUX4-rearranged B-other cases is shown. (b) STAP1 expression values are depicted as FPKM.

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