Figure 4
Quantitative real-time PCR results of ten AAs biosynthesis pathway genes in different tissues of N. pseudonarcissus ‘King Alfred’. Graphs are plotted using normalized ddCT values scaled to lowest. Histone was used for internal reference. Expression fold change and error bars were calculated using the comparative 2−ΔΔCt method72 from three independent experiments. Bars represent the mean standard deviation of three independent replicates. Abbreviations: Gene studied are PAL, phenylalanine ammonia lyase; C4H, trans-cinnamate hydroxylase; C3H, p-coumarate hydroxylase; 4CL, 4-hydroxycinnamoyl CoA ligase; HCT, hydroxycinnamoyl transferase; TYDC, tyramine decarboxylase; N4OMT, norbelladine 4-O-methyltransferase; CYP96T, noroxomaritidine synthase and NR, noroxomaritidine reductase. Tissues of N. pseudonarcissus ‘King Alfred’ are BB, bulb; RT, root; ST, stem; LF, leaf; FL, flower.
