Table 4 PCR and pyrosequencing primers for the detection of 8 significant SNPs.
SNP | Sequence | |
|---|---|---|
rs9923231 | Forward | 5′-GGGAAGTCAAGCAAGAGAAGACCT-3′ |
Reverse | 5′-bio-GCCTCCAGGGTTCAAGTGG-3′ | |
Sequencing | 5′-CCTGAAAAACAACCATTG-3′ | |
rs2306168 | Forward | 5′-ATACTTGCCCACCTTCATTGC-3′ |
Reverse | 5′-bio-CAGGGTTAAAGCCGTCCAA-3′ | |
Sequencing | 5′-CTGGGCTGGAGCTGT-3′ | |
rs2257212 | Forward | 5′-TGCAGTGCTCACACTCATGATAG-3′ |
Reverse | 5′-bio-TCAAACAACGGGATGAAGATAA-3′ | |
Sequencing | 5′-TGGTGTTACAGGTTCTAAAT-3′ | |
rs1143671 | Forward | 5′-TGACATGTGTTCTTTGCTCTAAGG-3′ |
Reverse | 5′-bio-TTGTTTTCATTTCCCACCACTG-3′ | |
Sequencing | 5′-AAATGGCCCCAGCCC-3′ | |
rs1143672 | Forward | 5′-CGTGAAGATGGGAACAGTATCTC-3′ |
Reverse | 5′-bio-GAATGCAGCCCCAAAGATTA-3′ | |
Sequencing | 5′-GGTAAAGGATACAGAAAGC-3′ | |
rs4149056 | Forward | 5′-bio-AAGGAATCTGGGTCATACATGTGG-3′ |
Reverse | 5′-GCGAAATCATCAATGTAAGAAAGC-3′ | |
Sequencing | 5′-AAGCATATTACCCATGAAC-3′ | |
rs4149117 | Forward | 5′-bio-GTTGTCTCCTTATGGGAACTGG-3′ |
Reverse | 5′-GCTGATAATAAATGGCTCAGAGCT-3′ | |
Sequencing | 5′-CCCATGAAGAAATGTGGTA-3′ | |
rs7311358 | Forward | 5′-AGTCATTGGCTTTGCACTGG-3′ |
Reverse | 5′-bio-GAAGAATGGTGTCCTGCACTTAA-3′ | |
Sequencing | 5′-GGATCTCTGTTTGCTAAAAT-3′ |
