Table 1 The CRISPR targeting sequences design based on the Optimized CRISPR Design web tool.
From: AICAR suppresses TNF-α-induced complement factor B in RPE cells
AMPKα1 | Sequence | Score | Targeted exon |
|---|---|---|---|
Guide #1 | 5′-CACCGAAGATCGGCCACTACATTC-3′ | 90% | 1 |
Guide #2 | 5′-CACCGATTCGGAGCCTTGATGTGGT-3′ | 80% | 2 |
Guide #3 | 5′-CACCGCAGATGGTGTACTGATGACC-3′ | 74% | 3 |
AMPKα2 | Sequence | Score | Targeted exon |
Guide #1 | 5′-CACCGAAGATCGGACACTACGTGC-3′ | 94% | 1 |
Guide #2 | 5′-CACCGCTGGGCGACACGCTGG GCGT-3′ | ||
84% | 1 | ||
Guide #3 | 5′- CACCGATTCGCAGTTTAGATGTTGT -3′ | 76% | 2 |
AMPKα1 + α2 | Sequence | Score | Targeted exon |
Guide #1 | 5′-CACCGAAGATCGGCCACTACATTC-3′ | 90% | Exon 1 for AMPKα1 gene |
Guide #2 | 5′-CACCGATTCGGAGCCTTGATGTGGT-3′ | 80% | Exon 2 for AMPKα1 gene |
Guide #3 | 5′-CACCGAAGATCGGACACTACGTGC-3′ | 94% | Exon 1 for AMPKα2 gene |
Guide #4 | 5′-CACCGCTGGGCGACACGCTGGGCGT-3′ | 84% | Exon 1 for AMPKα2 gene |
