Figure 5
A rapid assay for Hsp90 imaging in clinical breast specimens. (a and b) Representative fluorescence images of an ultrasound-guided core-needle biopsy treated ex vivo with 100 µM HS-27 for one minute for an ER + /PR + tumor (a) and non-tumor (b) biopsy. Tissue types were determined by a trained pathologist using the histology images shown to the left of the fluorescence images. The top right corner of each tumor image shows the percentage of total tumor involvement in that region along with the percent cellularity. (c) Plotting integrated fluorescence as a function of percent cancer at each site (n = 96) demonstrates a positive and significant correlation between the percent invasive cancer and HS-27 uptake at site level. There is one data point outside the graph range that is not shown, but was included in the analysis. (d) Plotting the average integrated fluorescence for each biopsy stratified by tumor (n = 6) and non-tumor (n = 6) sites reveals a positive and significant correlation between the percent invasive cancer and HS-27 uptake at biopsy level. (E-H) PDFs created from (e) all non-tumor images from all biopsies (n = 45), (f) all tumor images from sites with between 25% and 50% tumor involvement (n = 7), (g) all tumor images from sites with between 50% and 75% tumor involvement (n = 7), and (h) all tumor images from sites with greater than 75% tumor involvement (n = 25). The dashed black line shows the mean tumor fluorescence from all images containing tumor regardless of percent tumor involvement. The number to the right of the dashed black line represents the percentage of pixels falling above the overall tumor mean for each case.
