Figure 2
Unbiased bioinformatics analysis of miR-124 targets. Next generation sequencing was performed on RNA extracted from primary motor neurons transfected with either miR-124 mimics (N = 3) or control mimics (N = 6). (a) A dendrogram depicting the hierarchical clustering of Pearson correlation coefficients for global gene expression from miR-124 overexpressing motor neurons (blue) and controls (black). (b) Enrichment landscape plot for all 876 7mer motifs complementary to canonical mouse miRNA seed regions, gained by Sylamer analysis45. Sorted 6500 mRNAs expressed in primary motor neurons, ranked from down- to up-regulated after overexpressing of miR-124, or control mimic. Assessment of over- and under-represented miRNA recognition sequences (seed-matches) for all known miRNAs, identified two enriched motifs, both matching the miR-124 ‘seed’ sequence (blue, 7mer–2; light blue, 7mer-1A). (c) Top seven terms (by gene count) from gene ontology (GO) analysis on all mRNAs significantly up- or down- regulated following miR-124 overexpression (corrected P-value < 0.05), using database for annotation, visualization and integrated discovery (DAVID) software46. This analysis revealed an enrichment for mitochondrial-related genes and is further described in Table 1.
