Figure 3
miR-124 overexpression impacts mitochondrial localization and function. (a) Confocal fluorescent micrographs of primary motor neurons, 72 hrs post transfection with scrambled control or miR-124 mimics, stained with MitoTracker Deep Red FM (green) and TMRE (red) to depict functional mitochondria. Merged signal (yellow) relative to nuclei (blue, Hoechst), scale bars, 20 μm. Oligomycin A (1 µM) or miR-124 overexpression diminished mitochondrial activity. Bar graphs quantification of fluorescent signal intensity in (b) axons and (c) soma. Quantification of 3 random positions per compartment; 9 neurons per condition; Data collected from three replicates and the study performed in > four independent experimental repeats. Averages ± SEM, one way ANOVA with post hock Neuman-Keuls. *P-value < 0.05, **p < 0.01, ***p < 0.001.
