Table 2 List of synthetic DNA and RNA oligos used in the study.
miRNA mimics, designed as dsRNA oligonucleotides r- RNA bases; r_ * - Phosphorothioated RNA base; m- 2’ O-methyl RNA base | ||
|---|---|---|
| Â | Anti- sense mimic | Sense mimic |
miR-142-3p | rU*rG*rUrArGrUrGrUrUrUrCrCrUrArCrUrUrUrArUmGmGrA | 5SpC3/rUmCrCmArUmArAmArGmUrAmGrGmArAmArCmArCmUrAmCA |
miR-9-5p | rU*rC*rUrUrUrGrGrUrUrArUrCrUrArGrCrUrGrUrAmUmGrA | mAmUrAmCrAmGrCmUrAmGrAmUrAmArCmCrAmArAmGA |
miR-10a-5p | rU*rA*rCrCrCrUrGrUrArGrArUrCrCrGrArArUrUrUmGmUrG | mCmArAmArUmUrCmGrGmArUmCrUmArCmArGmGrGmUA |
miR-29a-3p | rU*rA*rGrCrArCrCrArUrCrUrGrArArArUrCrGrGmUmUrA | mAmCrCmGrAmUrUmUrCmArGmArUmGrGmUrGmCrUA |
miR-30c-5p | rU*rG*rUrArArArCrArUrCrCrUrArCrArCrUrCrUrCmAmGrC | mUmGrAmGrAmGrUmGrUmArGmGrAmUrGmUrUmUrAmCA |
miR-124-3p | rU*rA*rArGrGrCrArCrGrCrGrGrUrGrArArUmGmCrC | mCmArUmUrCmArCmCrGmCrGmUrGmCrCmUrUA |
miR-135a-5p | rU*rA*rUrGrGrCrUrUrUrUrUrArUrUrCrCrUrArUrGmUmGrA | mAmCrAmUrAmGrGmArAmUrAmArAmArAmGrCmCrAmUA |
miR-138-5p | rA*rG*rCrUrGrGrUrGrUrUrGrUrGrArArUrCrArGrGmCmCrG | mGmCrCmUrGmArUmUrCmArCmArAmCrAmCrCmArGmCU |
miR-206-3p | rU*rG*rGrArArUrGrUrArArGrGrArArGrUrGrUrGmUmGrG | mAmCrAmCrAmCrUmUrCmCrUmUrAmCrAmUrUmCrCA |
miR-218-5p | rU*rU*rGrUrGrCrUrUrGrArUrCrUrArArCrCrAmUmGrU | mAmUrGmGrUmUrAmGrAmUrCmArAmGrCmArCmAA |
Control | rC*rG*rCrGrArCrUrArUrArCrGrCrGrCrArArUrArUmGmGrU | rAmCrCmArUmArUmUrGmCrGmCrGmUrAmUrAmGrUmCrGmCG |
Quantitative real time PCR DNA primers | ||
Gene | Forward | Reverse |
Cfl2 | TCTCGTCCCAGTGCCACCGA | ACTCCAGATGCCATAGTGCCCGC |
Hprt | CTGGTTAAGCAGTACAGCCCCAAA | TGGCCTGTATCCAACACTTCGAGA |
Vim | AAATGGCTCGTCACCTTCGT | AGAAATCCTGCTCTCCTCGC |
Ptbp1 | ATTCCGTGTGGTCACAGACA | GTCACTGGAAGGAGCTCAGG |
Mdk | CCAGGAGACCATCCGCG | TCCTTTTCCTTTCTTGGCTTTG |
miR-142-3p | TGTAGTGTTTCCTACTTTATGGA | Universal primer (Qiagen) |
miR-9-5p | TCTTTGGTTATCTAGCTGTATGA | Universal primer (Qiagen) |
miR-10a-5p | TACCCTGTAGATCCGAATTTGTG | Universal primer (Qiagen) |
miR-29a-3p | TAGCACCATCTGAAATCGGTTA | Universal primer (Qiagen) |
miR-30c-5p | TGTAAACATCCTACACTCTCAGC | Universal primer (Qiagen) |
miR-124-3p | TAAGGCACGCGGTGAATGCC | Universal primer (Qiagen) |
miR-135a-5p | TATGGCTTTTATTCCTATGTGA | Universal primer (Qiagen) |
miR-138-5p | AGCTGGTGTTGTGAATCAGGCCG | Universal primer (Qiagen) |
miR-206-3p | TGGAATGTAAGGAAGTGTGTGG | Universal primer (Qiagen) |
miR-218-5p | TTGTGCTTGATCTAACCATGT | Universal primer (Qiagen) |
RNU6B (U6) | GATGACACGCAAATTCGTGAA | Universal primer (Qiagen) |
