Figure 1
Inactivation of Huwe1 in the differentiating spermatogonia leads to an arrest in spermatogenesis. (a) Inactivation of Huwe1 in the differentiating spermatogonia leads to infertility. WT and Stra8-Cre KO (n = 5) male mice were mated with CD1 females for 4 consecutive weeks. The females were changed every week. Indicated are the frequency of vaginal plugging, number of litters sired and the average litter size. (b)Adult Stra8-Cre KO mice have smaller testes than WT mice. Representative images (left panel) and weights (right panel) of testis from WT (n = 9) and Stra8-Cre KO (n = 10) mice. (c) Arrested spermatogenesis in the Stra8-Cre KO testis. Hematoxylin and eosin stained testicular tissue sections from adult (4 month old) WT and Stra8-Cre KO testes. Scale bar = 90 μm. (d) Few sperm in the Stra8-Cre KO epididymis. Hematoxylin and eosin stained caput (head of the epididymis) tissue sections from adult WT and Stra8-Cre KO. Scale bar = 90 μm. (e) Low sperm number in the Stra8-Cre KO mice. Measurement of sperm number in the cauda (tail of the epididymis) of WT and Stra8-Cre KO (n = 5) mice. Data are shown as mean ± SEM. Student’s t-test. ***p < 0.001.
