Table 2 Antibody information.

From: Ubiquitin Ligase Huwe1 Modulates Spermatogenesis by Regulating Spermatogonial Differentiation and Entry into Meiosis

Protein name

Company

Catalog #

Conc. for immunostaining

Stra8

Dr. Griswold, Washington State University

NA

1:2000

BrdU

Roche

11 170 376 001

1:50

γH2Ax

Abcam

ab11174

1:2000

γ-H2Ax

EMD Millipore

05–636

1:500

Sycp3

Abcam

ab15093

1:100

FK2

EMD Millipore

04–263

1:100

pCHK2

Cell Signaling Technology

2661 S

1:100

Cleaved Caspase 3

Cell Signaling Technology

9661

1:100

p53 (phospho S15)

Abcam

ab1431

1:50