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Figure 2

From: Misato underlies visceral myopathy in Drosophila

Figure 2

Visceral muscle is critical locus for the mst RNAi-induced phenotypes. (A) Schematic drawing of Drosophila visceral muscle comprised of the binucleated circular and the multinucleated longitudinal muscles. (B) Confocal images of the binucleated circular and the multinucleated longitudinal muscles expressing mCD8GFP with arrows indicating the nucleus in a muscle cell. Scale bars: 20 and 100 μm. (C) Vertical section of the confocal image of the intestine expressing mef2 > mCD8GFP stained with Hoechst and phalloidin. Note that mef2-GAL4 driven GFP was localized only at the exterior layer of visceral muscle. Scale bar: 50 μm. (D) Confocal images of the visceral muscles expressing mef2 > mCD8GFP with indicated genotypes stained with phalloidin. Scale bars: 100 μm. (E) Confocal images of the intestine expressing mCD8GFP driven by mef2-, how-, 5053A- and myo1A-GAL4. Scale bars: 200 μm. (F) Bright field and confocal images of the intestine with indicated genotypes. Insets are the zoomed-in images of the intestine stained with phalloidin. Scale bars: 200 μm.

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