Figure 4

Exaggerated expression of mst produces intestine with reduced diameter, potentiated visceral muscle layer and thickened muscle fibers, and increased food transportation along the intestine and excretion. (A) Bright field and confocal images of the intestine bearing indicated genotypes stained with phalloidin. Scale bars: 200 μm. (B) Comparison of diameter of the intestine with indicated genotypes. N = 5–11. ***p < 0.0001; *p < 0.05 by ANOVA Dunnett’s multiple comparison test. (C) Horizontal section and high magnification of confocal images of the intestine bearing indicated genotypes stained with phalloidin. Scale bars: 100 and 5 μm. (D) Comparison of the fecal spots of the flies with indicated genotypes. N = 7. ***p < 0.0001; **p < 0.001 by ANOVA Dunnett’s multiple comparison test. (E) Bright field images of the intestine of the flies with indicated genotypes fed on colored food. Dotted lines denote the midgut. Scale bar: 200 μm.