Figure 5
Visceral tubulin levels are altered by manipulation of mst expression. (A) Confocal images of visceral muscles with indicated genotypes stained with Hoechst, anti-tubulin antibody and phalloidin. Scale bars: 10 μm. White squares indicate the regions for the zoom-in images shown in the second rows of each genotype. (B) Immunoblot analysis of the level of tubulin and Mst expression using lysates from intestines of fifteen flies with indicated genotypes. These gel images were cropped from the original images with modified contrast and brightness for clarification. See Figure S3 for the raw gel images. (C) Confocal images of visceral muscles with indicated genotypes stained with Hoechst and anti-Mst antibody. Scale bars: 10 μm. (D) Bright field and confocal images of the intestine with indicated genotypes. Scale bars: 200 μm.
