Figure 6
Mst is highly expressed in visceral actin filaments and involved in actin-myosin structures. (A) Comparison of the level of mRNA expression in the throax and intestine with indicated genotypes. N = 15. ***p = 0.0004 by ANOVA Dunnett’s multiple comparison test. (B) Confocal images of visceral muscle expressing mef2 > mstGFP stained with Hoechst, phalloidin and anti-myosin antibody. Scale bars: 10 μm. White square and the inset illustrate the dense localization (arrowheads) of mstGFP in the segments of muscle fibers. (C) Confocal images of the visceral muscle with indicated genotypes stained with phalloidin. Scale bars: 10 and 5 μm. Note the arrows indicating the segments of muscle fibers that are lacked by mst depletion. (D) Super-resolution images of the actin filaments using OMX microscopy and a cartoon illustrating the joint of actin filaments in control and mst-depleted visceral muscles. Scale bars: 1 μm. (E) Confocal images of the visceral muscle with indicated genotypes stained with Hoechst, phalloidin and anti-myosin antibody. Scale bars: 10 μm.
