Figure 9
Visceral muscle apoptosis is increased along the progression of VM induced by mst depletion. (A) Confocal images of the visceral muscle with indicated genotypes expressing mef2 > mCD8GFP stained with Hoechst and TUNEL apoptosis detecting method. Scale bars: 20 μm. (B) Bright field and confocal images of the intestine stained with phalloidin dissected from the flies with indicated genotypes reared at 30 °C. Scale bars: 200 μm. (C) Bright field and confocal images of the intestine with indicated genotypes stained with phalloidin. Scale bars: 200 μm. (D) Comparison of diameter of the intestine with indicated genotypes. N = 8–11. ***p < 0.0001; n.s. not significant by ANOVA Dunnett’s multiple comparison test. (E) Comparison of contraction frequencies of the intestine with indicated genotypes. N = 10. ***p = 0.0002; n.s. not significant by ANOVA Dunnett’s multiple comparison test. (F) Comparison of the level of food intake by the flies with indicated genotypes. N = 6. ***p = 0.0002; n.s. not significant by ANOVA Dunnett’s multiple comparison test. (G) Comparison of the survivorship of the flies with indicated genotypes. N = 5–6. Statistical significance was analyzed by Log-rank (Mantel-Cox) test. p < 0.0001.
