Figure 2
Changes in fasting blood glucose, serum insulin, and triglyceride levels, body weight, percentage of body fat, and lean muscle mass of healthy and diabetic male and female rats. Six-hour fasting blood collection at the indicated ages was performed for analysis of (a) plasma glucose, (b) serum insulin, and (f) serum triglyceride levels using commercially available assays (Beckman-Coulter, Brea, CA) on an automated clinical chemistry instrument (AU680, Beckman-Coulter, Brea, CA). Insulin was measured by an ELISA kit specific for rat insulin. (c) Body weight was determined weekly and monthly. (d and e) Body composition measurements were performed using the EchoMRI 4in1/1100. Values are means ± SEM. n = 6 for ZL-F, ZDF-F, and ZDF-M, and n = 8 for ZL-M. *p < 0.05 vs. ZL-F, †p < 0.05 vs. ZL-M, and #p < 0.05 vs. ZDF-F using two-way ANOVA.
